FastQCFastQC Report
Sun 14 Apr 2019
SRR6576069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054474
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16720.15856246811206345No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16210.15372593349859742No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12900.12233587551708244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17500.059.64541
GGTATCA5550.059.3016931
ATCAACG23700.043.0214463
TCAACGC23750.042.9308744
CAACGCA24150.042.2198075
AACGCAG25350.040.2212336
TATCAAC29900.034.9095152
CTTAGGT5050.034.4257933
GTCTTAG7400.034.3102651
AGGTATA5150.033.757336
ACGCAGA30600.033.1669857
CGCAGAG31400.032.3219648
TAGGTAT5500.031.609145
GGTATAG5800.029.9741827
GTACATG39100.029.5815121
GCAGAGT35000.029.1316669
GTATCGA650.006142584628.9340131
ACATGGG40000.028.8967343
TACATGG40500.028.558942
GGTAATC1652.9722287E-928.4767048
Produced by FastQC (version 0.11.2)