FastQCFastQC Report
Sun 14 Apr 2019
SRR6576069_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576069_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054474
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17940.17013221757957048No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16620.157614127991776No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12020.11399048245855278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19700.060.226511
GGTATCA5850.057.9468731
ATCAACG25650.044.885653
TCAACGC26050.044.1964344
CAACGCA26500.043.800595
AACGCAG27500.042.378726
TATCAAC30850.037.778612
GTCTTAG6550.035.2215231
CGCAGAG33600.034.4297378
ACGCAGA33850.034.0123187
TAGGTAT4750.033.636765
CTTAGGT5400.033.0688173
AGGTATA5150.031.0241936
GCAGAGT37950.030.4832449
GGTATAG5400.029.587897
GTACATG40950.029.5483061
TTAGGTA5600.029.370334
ACCTAAG6750.028.5978551
TCTTAGG8300.027.7438952
TACATGG42750.027.3723872
Produced by FastQC (version 0.11.2)