FastQCFastQC Report
Sun 14 Apr 2019
SRR6576070_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576070_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276897
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8370.3022784645554123No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5170.18671202649360594No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5000.18057255947157247No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4690.16937706078433498No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4610.1664878998327898No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4370.15782041697815433No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4270.15420896578872287No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4040.14590262805303056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.13217911353319106No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3650.1318179684142479No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3500.12640079163010073No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3500.12640079163010073No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3260.11773330877546526No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3240.11701101853757896No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3210.11592758318074953No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3150.11376071246709066No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3020.10906582592082976No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2970.10726010032611404No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2920.10545437473139833No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2890.10437093937456889No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2880.10400979425562575No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2810.10148177842302372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT853.9461702E-738.699644
ACTGATA500.001695115437.621118
TATTAGG550.002715611834.17633
AATACTG753.2722673E-431.3282765
GTACATG20300.030.391021
TACATGG20550.029.9562342
ACATGGG20950.029.1599713
CTATTAG650.006151060628.918412
ATGGGAT1154.1865405E-628.604085
GTCCTAA856.723498E-427.7026351
GTATCAA12800.027.5944231
ATGGGAG3150.026.852815
ATGGGTG1755.524271E-926.8528085
CTAGCAC700.00883159926.8528083
GTAAGAG909.508326E-426.10693
AACGCAG13350.025.6962286
TAAGAGG2800.025.1745074
TAAGACA1701.2386772E-724.8783384
ATACTGA950.00130331224.732856
AGAGAGT950.00130331224.732856
Produced by FastQC (version 0.11.2)