FastQCFastQC Report
Sun 14 Apr 2019
SRR6576076_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576076_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309694
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16460.12567821185712083No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT15210.11613399771244276No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14620.1116291286361547No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT14400.10994934694669137No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14300.10918580981511712No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13360.1020085607783192No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13170.10055784022832814No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13170.10055784022832814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.060.173761
GGTATCA7450.058.7691
TCAACGC29400.044.2748834
ATCAACG29800.043.680593
CAACGCA30600.042.6921845
AACGCAG31450.041.538346
ACGCAGA37750.034.6061137
TATCAAC38750.034.198132
CGCAGAG39550.032.9349378
TACATGG53050.029.6745682
GTACATG53000.029.5794411
ACATGGG54100.029.2723543
GCAGAGT44700.029.1404199
GCGTAGT856.750511E-427.693171
CTAAGAC5650.025.7832873
TAAGACA6250.025.5637114
ATAGGGT2101.4333636E-924.6149163
AGAGTAC39350.024.31886110-11
TATAAGC2151.882654E-924.0424772
ACCTAAG3350.023.8905261