FastQCFastQC Report
Sun 14 Apr 2019
SRR6576077_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576077_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727304
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC22120.30413692211234916No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17170.236077348674007No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14340.1971665218395609No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14010.1926292169436714No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA11910.16375545851528384No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11820.16251801172549582No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10160.1396939931582942No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10010.1376315818419808No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA9210.12663205482164266No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC9170.12608207847062575No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8820.12126978539922784No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT8800.12099479722371938No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT8230.11315763422172848No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8080.11109522290541507No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC8000.10999527020338126No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC7960.10944529385236434No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7880.10834534115033055No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7450.1024330953768988No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA7370.10133314267486498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC200.002163205570.495513
GTATCAA19550.053.025181
ATCAACG24200.041.753543
CAACGCA24450.041.326615
AACGCAG29000.036.301146
GTACTAG5750.036.0571251
GGTATCA8550.035.271191
TATCAAC29400.035.167832
ACGCAGA29000.035.004677
CGCAGAG28950.034.7404488
TCAACGC29500.034.5706834
TAACCAC2050.034.3880546
GTATATA1800.034.031191
GCAGAGT30150.033.5136229
TAGGCAT6500.032.536395
TAGTACT8100.029.5907124
ATAACCA2750.029.0526965
CTAGGCA7650.028.873984
TACGGGC5250.028.64584
TATTCTC3450.028.6068765
Produced by FastQC (version 0.11.2)