FastQCFastQC Report
Sun 14 Apr 2019
SRR6576078_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576078_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662006
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC19410.2931997595187959No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT15240.2302093938725631No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA13660.206342540702048No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13270.20045135542578166No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11960.18066301513883562No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10710.16178101104823822No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG9620.14531590348123732No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC9380.14169055869584263No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC9010.13610148548502582No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC8870.1339867010268789No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8450.12764234765243818No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT8390.1267360114560895No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC7850.11857898568895146No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT7720.11661525726352932No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC7540.11389624867448331No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7280.10996879182363906No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT7210.10891139959456561No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7160.10815611943094171No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7100.10724978323459304No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC6840.10332232638374879No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA6650.10045226176197798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17950.050.3824231
CAACGCA21900.039.911045
ATCAACG22250.039.2832263
CGTTAAC1450.038.9812281
AACGCAG26000.035.6055036
ACGCAGA25600.034.142657
AGGGCTA5650.034.100415
CGCAGAG25950.033.5263868
GTACTAG3950.033.3889851
TAACAGT2000.032.894424
TATCAAC28150.032.3852732
GCAGAGT27300.031.8684849
TCAACGC27900.031.4964524
TTAACAG2051.8189894E-1229.7998263
GTATAAC650.006087286428.986041
TAGTACT6650.028.972534
CTAACGC2800.028.5308763
ACATGGG28150.028.0449773
TCTAACG2850.028.0303332
TAACCAC1354.6365676E-727.8471285
Produced by FastQC (version 0.11.2)