FastQCFastQC Report
Sun 14 Apr 2019
SRR6576079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712030
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20770.1213179675589797No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18760.10957751908553005No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18400.10747475219476295No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17800.1039701407101511No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17740.10361967956168992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.059.5788541
GTATCAA26400.058.402851
TCAACGC34200.044.1130334
ATCAACG34900.043.093583
CAACGCA36700.041.492255
AACGCAG37900.040.4265256
TATCAAC44600.033.8325162
ACGCAGA48750.031.2362147
AGGTATA5800.030.7924046
CGCAGAG50900.029.9168058
TAGGTAT6000.029.7659915
GTACATG62600.029.7361641
GTCTTAG10300.029.6647171
ACCTAAG7300.029.6209561
GGTATAG6250.029.3273377
ACATGGG63600.028.82013
TACATGG65150.028.4279772
CTTAGGT6400.027.9056193
GCAGAGT56000.027.5279469
TAAGACA13100.026.5490174
Produced by FastQC (version 0.11.2)