FastQCFastQC Report
Sun 14 Apr 2019
SRR6576079_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576079_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712030
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA19430.11349100191001325No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19290.1126732592302705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26850.059.8788071
GGTATCA9550.054.800271
ATCAACG35750.043.7796063
TCAACGC35800.043.718464
CAACGCA37700.041.63865
AACGCAG39500.039.5031666
TATCAAC46100.034.5622862
ACGCAGA50150.031.1123457
CGCAGAG52500.029.630188
ACCTAAG8050.028.6987691
GTACATG68100.028.662651
TACATGG68500.027.9259192
ACATGGG69300.027.6035423
GCAGAGT59500.026.2232639
TAAGACA12950.026.1316224
CTAAGAC12600.025.738443
GTACCGT1501.1632474E-625.0662696
AGAGTAC50450.023.66127410-11
CATGGGG41500.023.3304334
ATTATCC9500.022.75823
Produced by FastQC (version 0.11.2)