FastQCFastQC Report
Sun 14 Apr 2019
SRR6576081_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576081_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621016
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8970.14444072294433638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.137194532830072No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6220.1001584500238319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16900.044.051541
ATCAACG21250.033.846033
GTACATG36000.033.375551
TCAACGC22000.032.6895564
TACATGG36100.032.6844982
CCCTTAT1308.951247E-932.6204451
CAACGCA22200.032.3950585
AACGCAG22750.032.231726
ACATGGG37050.030.958283
TATCAAC24200.029.9144212
CATGGGA22450.027.4280684
ACGCAGA26700.027.1068977
CGCAGAG27100.026.7067978
ATGGGAG11000.026.0661835
CATGGGG12050.025.7452554
GCAGAGT31400.023.0494969
GGTATAC1100.003013656721.4174651
GGTATCA8600.021.3676571
GTATAGT1805.5358396E-620.941521
AGAGTAC29550.020.59598710-11
Produced by FastQC (version 0.11.2)