FastQCFastQC Report
Sun 14 Apr 2019
SRR6576082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235050
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17820.14428565645115582No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17150.13886077486741427No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17080.13829399619448604No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14510.11748512205983563No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14210.11505607060442898No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13820.11189830371240031No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13000.10525889640095541No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12720.10299178170924254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7600.064.33251
GTATCAA23500.060.0155871
TCAACGC31850.043.5311474
ATCAACG32050.043.2595063
CAACGCA33900.040.8987355
AACGCAG34500.040.1874546
TAGGTAT5600.037.7669645
AGGTATA5400.036.554696
TATCAAC39200.035.8588032
GTCTTAG7600.034.6405751
CTTAGGT5950.033.965583
ACGCAGA41650.033.288537
GGTATAG5850.032.9393927
TTAGGTA6350.031.8260154
TATACCG1052.0659118E-631.332594
CGCAGAG44700.030.9120188
GTATAGT6500.029.6454528
TCTTAGG7650.029.4978582
GTACATG52700.028.4571061
GCAGAGT48600.028.4314259
Produced by FastQC (version 0.11.2)