FastQCFastQC Report
Sun 14 Apr 2019
SRR6576083_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576083_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108199
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18400.1660351615549193No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC16900.15249968642815956No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15540.14022752231323074No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13620.12290211415097829No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12370.1116225515453452No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC12080.10900569302083832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21450.059.4577371
GGTATCA7600.058.2077261
TCAACGC28200.044.1586724
ATCAACG28950.043.3393063
CAACGCA29950.041.578455
AACGCAG31550.040.0656476
TATCAAC32900.037.8502922
ACGCAGA37550.033.1631057
CGCAGAG39750.031.231998
GTCTTAG5300.029.3024851
GTACATG49150.029.2040251
GCAGAGT44400.028.0669889
TACATGG51300.027.6636092
TAGGTAT4300.027.3206395
ACATGGG51000.027.2734973
CTTAGGT4200.026.8522833
GTATATG1659.199721E-825.6699451
TTAGGTA4450.025.3437294
TAAGACA10050.024.7815864
ATGGGAG12350.024.7323655
Produced by FastQC (version 0.11.2)