FastQCFastQC Report
Sun 14 Apr 2019
SRR6576084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557201
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA10890.1954411424243675No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC9460.16977715402520813No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8610.15452233574598753No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8140.14608731857983026No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8040.14429263407639253No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7940.1424979495729548No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7800.13998539126814202No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7630.1369344276122979No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7070.12688419439304668No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6830.12257695158479616No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT6780.12167960933307728No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.12114120398204598No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC6490.11647502427310791No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG6490.11647502427310791No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG6340.11378299751795132No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC6230.11180884456416984No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6190.11109097076279476No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC6110.10965522316004458No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6060.10875788090832572No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5970.10714266485523176No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5850.1049890434511065No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5700.10229701669594994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15900.056.181971
GGTATCA6550.043.0675771
ATCAACG21600.041.123743
CAACGCA22050.040.2844855
TCAACGC22800.039.165474
AACGCAG23750.038.1925166
CTAACGC1401.2732926E-1136.9274443
TCTAACG1353.43789E-1034.8262522
ACGCAGA26000.034.3451047
CGCAGAG26300.033.5959328
TATCAAC27250.032.953942
GCAGAGT27950.031.780789
ACGTTAT753.2670924E-431.3436281
TAACACT753.2739784E-431.3323764
CGCCTAC1602.1300366E-929.3741027
CTTAGGT804.7786138E-429.3741023
TATTCTC3800.028.44655
ACCTAAG1205.8212136E-627.4256741
CTGTGCG4850.027.1331929
GTACAAT700.00881804726.8659651
Produced by FastQC (version 0.11.2)