FastQCFastQC Report
Sun 14 Apr 2019
SRR6576084_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576084_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557201
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA11060.19849210608021162No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8510.15272765124254983No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT7720.13854964366539185No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7530.13513974310886018No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7400.13280665325439114No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7180.12885834734682816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.12329482538617124No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6760.12132067243238974No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT6670.1197054563792958No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6490.11647502427310791No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT6460.1159366189220766No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC6030.10821947555729441No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5910.10606585415316913No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5640.10122020599388731No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5580.10014339529182469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16850.051.7426641
GGTATCA6800.047.8208351
CAACGCA20550.041.8548165
ATCAACG20600.041.7532233
AACGCAG22550.039.6016466
TCAACGC22400.038.3980524
TAGGCAT3150.037.30235
CGCAGAG23900.036.5748258
ACGCAGA23950.036.4984667
GTACTAG3150.035.906881
ATATCGC801.1574273E-535.2506756
TAACCAC1405.0931703E-1033.5720676
TATCAAC26100.033.134732
GCAGAGT26250.033.121479
CACTGCG753.274749E-431.331127
CTAGGCA3850.030.5200614
GTACATG25250.029.4898571
GAGTTGC4050.029.01290194
GAACCGT650.006150909228.923636
ACATGGG24800.028.2384433
Produced by FastQC (version 0.11.2)