FastQCFastQC Report
Sun 14 Apr 2019
SRR6576085_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576085_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496047
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA11180.22538186905676277No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7720.15563041405350703No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7050.14212362941414827No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6900.13909972240533658No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6700.1350678463935877No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA6580.13264872078653836No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6440.12982640757831415No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT6330.12760887577185226No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC6210.12518975016480294No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5850.11793237334365493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.1122877469272065No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5560.11208615312661904No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5540.11168296552544416No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC5520.11127977792426927No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5450.10986862132015716No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5250.10583674530840828No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC5250.10583674530840828No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA5220.10523196390664594No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5160.10402240110312128No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG5130.10341761970135895No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC5110.10301443210018406No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4970.10019211889195984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.054.283561
TGTACGA558.804054E-751.2699782
GTATCAA15050.049.101531
CGTGTAC555.2835494E-542.7206733
TACGATC450.001014168141.7713284
GTGTACG608.7413115E-539.2238851
CAACGCA18900.037.7931025
AACGCAG19500.037.112226
ATCAACG19350.036.9141963
TCAACGC20550.035.215974
TAAACGT550.002717583434.176544
ACCTAAG1256.162736E-933.8894351
TGATCGC1457.366907E-1032.431679
ACGCAGA22300.032.2416577
TATCAAC23050.031.6035122
GTTCGGA753.26038E-431.35377994
CGCAGAG23000.031.0780038
GCAGAGT24250.029.4760469
GTACGAT804.7813324E-429.3704623
GGGCTAT5500.029.050066
Produced by FastQC (version 0.11.2)