FastQCFastQC Report
Sun 14 Apr 2019
SRR6576086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499206
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17710.11812919638795469No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17660.11779568651672952No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA17500.11672845492880898No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16680.11125889304071622No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16350.10905772789063012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8250.063.831431
GTATCAA24500.058.9172941
TCAACGC32950.042.790564
ATCAACG33400.042.3547553
CAACGCA34500.041.004315
AACGCAG35700.040.0209586
TATCAAC41450.034.937892
ACGCAGA45050.031.61047
CGCAGAG47300.030.1067338
GCGTTAG650.00614281828.9345741
GTACATG58500.028.8542021
TACATGG59700.027.8804262
GCAGAGT51650.027.5711219
TCTAGAC2253.6379788E-1227.1545733
ACATGGG61050.027.0981183
TTAGGTA4000.027.0240214
GTCTTAG7100.025.1649281
AGAGTAC44850.023.4729510-11
CTTAGGT4500.022.9769463
TATAGCG1100.003060615421.3628645
Produced by FastQC (version 0.11.2)