FastQCFastQC Report
Sun 14 Apr 2019
SRR6576087_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576087_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334776
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16140.12091916546296906No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15210.11395170425599502No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14390.10780835136382433No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13620.10203959316020066No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13460.1008408901568503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.059.15571
GTATCAA22050.055.715661
TCAACGC27700.043.769234
ATCAACG28250.042.750743
CAACGCA29450.041.4874765
AACGCAG30700.039.798256
TATCAAC36100.033.71742
ACGCAGA39600.030.8536917
GTACATG50550.030.7282731
TACATGG51200.030.5658022
CGCAGAG41050.029.7839368
ACATGGG52400.028.966783
GCAGAGT44500.027.4748429
TAGGTAT3250.027.4725655
CCCTATA1205.847911E-627.4143712
GTCTTAG5700.027.2511271
CTTAGGT3550.025.1509423
AGGTATA3450.024.5178496
AGAGTAC38300.024.43296610-11
TACGTGT2957.2759576E-1222.3015464
Produced by FastQC (version 0.11.2)