FastQCFastQC Report
Sun 14 Apr 2019
SRR6576088_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576088_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085015
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC21250.1958498269609176No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT18630.1717026953544421No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15580.14359248489652215No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14980.13806260742939036No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA14890.13723312580932062No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13870.12783233411519657No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13870.12783233411519657No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13430.12377709063929991No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13150.12119648115463842No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12740.11741773155209836No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12410.11437629894517588No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12280.11317815882729731No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11750.10829343373133091No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11500.105989318120026No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT11330.10442251950433866No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11290.10405386100652986No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11090.10221056851748593No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA10860.10009078215508542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24250.054.9728471
CAACGCA30500.042.37845
ATCAACG31300.041.5955773
AACGCAG35600.037.891666
GTACTAG4650.036.4689371
GGTATCA9950.036.4536671
TCTAACG2200.036.3193362
TCAACGC37650.034.580124
ACGCAGA37750.034.2347687
CTAACGC2700.033.0751273
CGCAGAG39200.032.8485458
TATCAAC40400.032.8079722
GCAGAGT42050.030.7339489
GTACATG44100.027.6652581
TACATGG44850.027.0376512
AGAGTAC37150.026.75489210-11
ACATGGG45400.026.7101023
CTAGGCA7150.025.6371824
TAGGCAT6800.025.5743475
CATGGGG28550.025.3528234
Produced by FastQC (version 0.11.2)