FastQCFastQC Report
Sun 14 Apr 2019
SRR6576092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566928
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9090.16033782067564137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.11624051025879828No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5780.10195298168374115No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5740.10124742471707165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.042.6738171
GTACATG33950.035.857991
ACATGGG33150.035.434743
TACATGG34050.035.2005162
ATCAACG19050.033.2974933
AACGCAG19150.032.6328966
CAACGCA19550.032.4458925
TCAACGC19850.031.9555264
CATGGGA18350.030.4707834
GTAATAC1102.971612E-629.9004753
TATCAAC22100.028.9249532
ACGCAGA22750.027.4690077
CGCAGAG23200.026.9362058
ATGGGAG8800.026.6968525
CATGGGG11750.025.5926274
GTGTAAG2051.0786607E-925.2211461
GCAGAGT26950.023.362479
CATGGGC6550.022.955224
CATGGGT8150.022.4843184
GTTAGTA1100.003056504321.3650192
Produced by FastQC (version 0.11.2)