FastQCFastQC Report
Sun 14 Apr 2019
SRR6576092_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576092_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566928
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8780.1548697541839528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.1365252730505461No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5940.10477520955041911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG408.002466E-658.78244494
GTATCAA16900.042.0729831
TACATGG32300.034.7717362
GTACATG32800.034.598421
ACATGGG33900.033.1305923
CAACGCA21950.031.4740125
AACGCAG22100.031.4730436
ATCAACG22000.031.3997143
TCAACGC22450.030.770324
TATCAAC25800.027.3213752
CATGGGA20350.027.2488784
ACGCAGA26100.026.647247
CGCAGAG26350.026.4107278
ATGGGAC4050.025.5291865
ATGGGTA4450.025.3466515
TAGTACT2051.0804797E-925.215634
CATGGGG12300.024.0694644
CATGGGT7350.023.0168674
ACTATAC1852.813722E-722.8613473
GCAGAGT30150.022.7700949
Produced by FastQC (version 0.11.2)