FastQCFastQC Report
Sun 14 Apr 2019
SRR6576096_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576096_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372970
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8070.21637128991607904No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6340.1699868622141191No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5790.15524036785800466No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC5450.14612435316513392No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4350.11663136445290506No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4070.10912405823524679No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG3760.1008123977799823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2800.062.1729431
GTATCAA7550.049.230921
ATCAACG8700.042.6718333
TCAACGC8850.041.9485784
CAACGCA9050.041.021545
AACGCAG9650.038.4709786
TATCAAC11200.033.146872
AATAACC1151.13530405E-732.6908042
ACCGTGC600.004146795331.34978
AGGTATA1701.2187229E-1030.4072536
ACGCAGA12350.030.0603187
GTATAAA1102.9370112E-629.9407831
GTGTATA804.7452407E-429.4061241
CGCAGAG12650.029.3671118
GTACATG15750.028.6779731
GGTACCT2650.028.392188
TACATGG15950.027.6949522
CAGTACC856.813302E-427.6429584
ACCTAAG2750.027.374431
ACATGGG16000.027.3146973
Produced by FastQC (version 0.11.2)