FastQCFastQC Report
Sun 14 Apr 2019
SRR6576101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853726
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20990.2458634269074621No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG15210.17816020596772267No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT14780.17312346115732682No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC14630.17136645715370036No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT13050.15285934831550171No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12880.15086807711139172No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12050.14114598829132532No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11340.13282950267416008No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11120.1302525634688413No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10730.1256843530594125No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10640.12463015065723664No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT10060.11783640184321433No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9710.11373672583475261No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9630.1127996570328185No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9440.11057411862822497No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT9080.10635730901952149No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC9010.10553737381782914No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8930.10460030501589504No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8570.10038349540719153No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8570.10038349540719153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20950.051.383221
CAACGCA24500.043.5465055
ATCAACG25750.041.4325943
AACGCAG28150.039.5697676
ACGCAGA29800.035.643947
TCAACGC30450.035.037424
CGTTAAC1900.034.637321
CGCAGAG30750.034.2370578
GCAGAGT31150.034.099189
TATCAAC32200.033.5769922
CCTTTAT1658.54925E-1131.3385281
CTAACTG3200.030.8434434
CTAAACG404.7821229E-429.37470862-63
GACGTTA1154.1950534E-628.6084127
CGGGCGT6300.028.3489256
ACCTAAG1704.0672603E-927.6516421
TAGGGTC856.8150397E-427.6467824
TAGTACT8200.027.5119234
GTATAAG5300.027.4951251
ACATGGG37300.026.8388753
Produced by FastQC (version 0.11.2)