FastQCFastQC Report
Sun 14 Apr 2019
SRR6576101_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576101_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853726
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC19590.2294647228736152No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT15580.18249414917666792No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC13160.1541478179181611No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT11520.13493790747851184No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC11410.13364943787585246No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG11150.13060396426956658No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10530.12334168105457721No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10200.11947627224659903No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9800.11479092823692848No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9660.11315105783354377No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9560.11197972183112614No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9200.10776291222242265No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9070.10624017541927971No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9030.10577164101831266No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8590.10061776260767506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17500.048.9924931
GTACTAG4400.042.8256071
CAACGCA21700.037.901465
ATCAACG22600.037.015973
TAGGCAT6700.036.4759065
CTAGGCA7300.032.1902774
GGTATCA8050.032.1857031
AACGCAG26700.031.8599346
AGGGCTA3850.031.7387775
TCAACGC26700.031.507894
CTATTGA3500.030.8842729
ACGCAGA27650.029.915427
TATCAAC29100.029.3938162
CGCAGAG28750.028.93438
GGGCTAT3900.028.9217266
GGCTATT3800.028.4460377
GTATAAG5650.028.3482781
GCAGAGT29600.028.1034169
TGGGTAC4200.027.9748846
TGATCGC5550.027.944649
Produced by FastQC (version 0.11.2)