FastQCFastQC Report
Sun 14 Apr 2019
SRR6576102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765389
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC18340.23961671777357657No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG13250.17311458617774753No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13170.17206936603478754No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC12450.16266238474814768No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11500.15025039555049785No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10880.14214993944255797No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10140.1324816531201781No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9520.12438119701223822No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA9450.12346662938714824No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9360.12229075672631824No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA9220.12046162147613829No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8900.11628074090429834No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8620.1126224704039384No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC8450.11040137760014843No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA8300.10844158983209845No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8230.10752702220700847No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8210.10726571717126847No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8010.1046526668138685No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC7880.10295418408155853No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT7760.10138635386711854No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7690.10047178624202856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15900.052.027151
ATCAACG19850.039.7668653
CAACGCA19900.039.4308365
AACGCAG23700.034.0998656
GGTATCA6900.034.0592731
ACGCAGA24100.032.3641057
CGATTGA300.004167288531.32429580-81
TCAACGC25650.031.1411154
TATCAAC26600.030.9202142
CGCAGAG25750.030.2902898
GCAGAGT26000.030.1797529
TAGTACT6950.029.7468134
GTACTGT7550.029.249846
CTGTGCG7400.029.207799
GACGTTA1303.3375727E-728.9147347
TCTAACG2300.028.6079182
TATTCTC2800.028.5274835
ACATGGG29300.027.742853
GTACATG30000.027.731061
ATGCATT856.825493E-427.6390846
Produced by FastQC (version 0.11.2)