FastQCFastQC Report
Sun 14 Apr 2019
SRR6576102_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576102_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765389
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC17730.2316469141835067No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT14860.19414964155481723No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC11400.14894387037179788No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT10670.139406236567288No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10470.13679318620988806No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG10430.13627057613840804No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC10110.1320896955665681No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9610.1255570696730682No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8720.11392899558263837No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8560.11183855529671839No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8550.1117079027788484No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8530.1114465977431084No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8440.11027072508227843No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8080.10556723443895849No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8070.1054365819210885No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7960.1039994042245185No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7900.10321548911729853No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA7870.10282353156368854No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7700.10060243875989856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACC602.5582267E-854.8234983
GTATCAA16150.048.6618461
ATCAACG20350.037.177613
GTACTAG3950.036.932541
CAACGCA20500.036.676355
GGTATCA7250.036.3491821
ACCTAAG2000.035.2944071
AACGCAG23600.033.6507456
TATCAAC26000.030.7292732
ACGCAGA24350.030.6844357
CGCAGAG25050.029.8464858
GTACATG31350.029.5714971
TCAACGC25800.029.32424
ACATGGG30300.029.3115753
GCAGAGT25900.028.8669669
CATGGGT5550.028.7876284
TACATGG31750.028.7167132
CTAGGCA6800.026.951054
GTGTTAC700.00878013226.8909761
GAGTATA700.00878013226.8909761
Produced by FastQC (version 0.11.2)