FastQCFastQC Report
Sun 14 Apr 2019
SRR6576103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518593
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14740.28423060087583135No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9070.17489630596633582No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8780.16930425208207592No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7910.1525280904292962No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7370.14211530043791568No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6380.12302518545371804No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6030.1162761549037492No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5990.11550483712660989No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5850.11280522490662234No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5590.1077916593552169No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5550.10702034157807762No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5350.10316375269238112No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC5340.10297092324809629No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5300.10219960547095698No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5230.10084979936096322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13200.040.2462431
GTACATG31700.034.1106031
TACATGG31850.033.9499552
ACATGGG31500.033.720433
CATGGGG15300.030.7187654
TCAACGC18050.029.6840294
ATCAACG17950.029.5875633
CAACGCA18050.029.4236435
AACGCAG18000.029.2442676
ATGGGCG856.811254E-427.6468895
TCGGTCT856.811254E-427.6468894
ATGGGGA7900.027.366925
ATGGGTA5400.026.9813145
GGCTAAT1606.8817826E-826.4449881
GTACTAG909.4948994E-426.1185051
CATGGGT8400.025.7379364
TATAAGG1659.271571E-825.6436212
ACGCAGA20600.025.5532427
CGCAGAG21150.025.1109568
GTCCTAC1000.001754489523.5066551
Produced by FastQC (version 0.11.2)