FastQCFastQC Report
Sun 14 Apr 2019
SRR6576103_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576103_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518593
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13640.26301936200450066No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8900.17161820541349382No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8350.16101258597782847No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7990.1540707259835748No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7480.14423642432504874No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7060.1361375876650861No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.12013274378944566No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6110.11781879045802779No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6010.11589049601517953No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5840.1126123954623375No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5750.11087693046377409No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5430.10470638824665972No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5350.10316375269238112No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5290.10200677602667217No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5260.1014282876938177No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5260.1014282876938177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.044.7510761
TAGATCG651.4129485E-436.151555
GTACATG30450.034.356591
AACGCAG18300.034.1562966
ACATGGG31050.033.299013
TCAACGC18650.033.2632984
TACATGG31250.033.2362862
CAACGCA18850.032.9103745
ATCAACG18800.032.747923
ATGGGTA6150.030.5671625
ACGCAGA20750.030.1233847
CATGGGT8500.029.8569264
CGCAGAG21550.029.0051168
CATGGGA12400.028.4256114
GGTACCT6450.027.6881628
GGTATCA7500.027.6462211
CGTCGTT350.00883837126.85543430-31
TGGGTAC7050.026.6649726
TATCAAC23450.026.655022
GGGTACC6850.026.0713337
Produced by FastQC (version 0.11.2)