FastQCFastQC Report
Sun 14 Apr 2019
SRR6576106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129514
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA34470.3051755002594036No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC31700.2806516785095183No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30110.266574827757779No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG14790.13094127208693296No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT14180.12554071928280658No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12330.10916199356537414No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11720.10376144076124774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21950.054.6166951
GGTATCA7200.054.1957661
TCAACGC27950.042.878894
ATCAACG28200.042.498763
CAACGCA28700.041.4308475
AACGCAG30100.039.659976
TATCAAC31600.037.9378622
ACGCAGA36650.032.44387
CGCAGAG39450.030.0219358
GCGTAGT650.006145689628.9311981
GTACATG47950.028.531471
GCAGAGT42400.028.1548399
TACATGG49850.027.1610832
ACCTAAG14150.026.9121481
GTCTTAG10550.026.737361
TTAGGTA9850.026.7200324
TAGGTAT9550.026.575145
CTTAGGT9750.026.5120473
ACATGGG51700.026.3628653
CTAAGAC16000.026.1429863
Produced by FastQC (version 0.11.2)