FastQCFastQC Report
Sun 14 Apr 2019
SRR6576106_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576106_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129514
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA39040.3456353794640881No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC33350.29525973117641746No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31800.28153701503478484No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG15450.13678449315369265No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13850.12261910874942675No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12680.1122606714038073No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC11620.10287610423598115No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11420.1011054311854479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20400.058.245331
GGTATCA6500.055.130381
ATCAACG25500.045.341843
TCAACGC25650.045.0766834
CAACGCA26600.043.2901045
AACGCAG27750.041.4961056
TATCAAC30250.039.154292
TAGGTAT9950.036.844765
TTAGGTA10050.036.4781464
GTCTTAG10900.036.3365361
AGGTATA10400.035.2505156
CTTAGGT10550.034.749323
GGTATAG10550.034.7462467
ACGCAGA33300.034.5770237
GTATAGT11350.033.1253058
TATAGTA12050.031.9810379
TCTTAGG11850.031.7304212
CGCAGAG37750.030.7500448
CTAAGAC13850.030.2026083
GTACATG46100.029.047461
Produced by FastQC (version 0.11.2)