FastQCFastQC Report
Sun 14 Apr 2019
SRR6576107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026413
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA35100.3419676095294974No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC32050.3122524753681023No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27910.2719178342441103No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT14120.13756645716685192No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG13520.13172085700395456No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG12390.12071164336383113No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11750.11447633652340725No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11660.11359949649897264No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT10620.10346712288328383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.055.5096781
GTATCAA18550.054.7256661
TATAGCG405.6843297E-446.9867485
TCAACGC22650.044.6032834
ATCAACG23150.043.436953
CAACGCA23650.042.9139025
AACGCAG24400.041.9799656
TTAGGTA9650.038.9546434
TAGGTAT10000.038.0592655
CTTAGGT9650.037.9807783
AGGTATA9900.037.969096
GTCTTAG10300.037.8723341
GGTATAG10450.036.4203537
TATCAAC27800.036.3475272
TCTTAGG10650.035.7451062
TATAGTA11000.034.5993359
ACGCAGA30100.034.030277
GTATAGT11450.033.64998
CGCAGAG33050.030.8506058
GTACATG40700.030.6007921