FastQCFastQC Report
Sun 14 Apr 2019
SRR6576107_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576107_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026413
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA37680.3671036902299562No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC32870.3202414622573954No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC29490.2873112480064068No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG14790.1440940440154207No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT13230.1288954835918875No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12800.1247061368084777No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11100.10814360301360175No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT11000.10716933631978551No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10890.10609764295658765No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10880.10600021628720602No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10800.10522080293215305No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT10440.10171344283441461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18800.058.350111
GGTATCA5800.056.8216251
ATCAACG23150.046.4838643
TCAACGC23300.046.1846124
CAACGCA24250.044.181545
AACGCAG25500.042.5686156
TATCAAC27400.039.445282
GTCTTAG10300.036.567591
TAGGTAT10000.034.303675
CTTAGGT9800.034.044743
AGGTATA10000.033.8337556
ACGCAGA32000.033.628177
TTAGGTA10300.032.8483054
CGCAGAG33350.032.006938
TCTTAGG12000.031.3275532
GGTATAG11200.030.6282797
ACCAGAT14750.029.33270894
GTATAGT11900.029.2414428
GCAGAGT36750.029.1736989
TATAGTA11800.029.0907449