FastQCFastQC Report
Sun 14 Apr 2019
SRR6576110_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576110_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314832
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA20060.1525670199690911No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15690.11933083466176668No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15100.11484356936855812No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC13210.10046910936150019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8000.060.667531
GTATCAA23500.060.1537131
ATCAACG29800.046.3677023
TCAACGC30800.045.014854
CAACGCA32000.043.3267945
AACGCAG33150.041.9655276
TATCAAC35650.039.1544762
ACGCAGA41150.033.577287
GTACATG57000.031.2482721
CGCAGAG44200.031.1539698
TACATGG58800.029.653852
ACATGGG59600.029.4135253
GCAGAGT47850.029.0721919
TATACGG1154.198E-628.6078175
GTCTTAG5800.028.434731
ACCTAAG7050.028.0717331
TCGATAA700.00883780226.8563165
CATGGGG35300.024.630974
AGAGTAC41400.023.8389410-11
CTAAGAC9100.023.7575113
Produced by FastQC (version 0.11.2)