FastQCFastQC Report
Sun 14 Apr 2019
SRR6576111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172509
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17400.14839971377618424No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16360.13952984582634334No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14400.12281355622856627No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC14240.12144896115935998No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13950.11897563259642356No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13630.11624644245801098No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA12710.10840002081007481No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12540.10695013854904313No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12240.10439152279428131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21900.059.4547461
GGTATCA7150.057.1957931
TCAACGC30500.042.0560464
ATCAACG30900.041.511633
CAACGCA31200.041.112485
AACGCAG32100.039.9597976
TATCAAC36600.035.318522
ACGCAGA37950.033.6761747
TAGGTAT4700.032.9899525
AGGTATA4850.031.9696486
CGCAGAG40600.031.5938288
CTTAGGT4800.031.3237933
GTACATG53250.031.2488841
GGTATAG5000.031.010567
TATATAC1859.094947E-1230.4772053
GCAGAGT43250.029.6580229
GTCTTAG7150.029.5840321
TACATGG55850.029.5416552
TTAGGTA5250.029.5338634
ACATGGG56600.028.7227043