FastQCFastQC Report
Sun 14 Apr 2019
SRR6576114_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576114_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473459
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13680.2889373736691033No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8390.17720647405583165No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8230.17382708956847373No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7890.16664589753283812No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7190.15186109040064716No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6600.13939961010351476No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6350.134119321842018No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6220.13137357194603969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.12714934133684228No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5560.1174336109356882No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5330.11257574573511117No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5310.11215332267419144No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5200.10982999583913286No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5150.10877393818683351No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5020.10602818829085518No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4940.10433849604717621No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4880.10307122686441698No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4870.1028600153339571No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4760.10053668849889852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.047.980281
TGGGTAC5300.037.2391746
ATCAACG17350.036.0229073
AACGCAG17800.035.904226
GTACATG26700.035.6274721
ATGGGTA5550.035.5617335
CAACGCA17750.035.4758725
TCAACGC17950.034.81884
TACATGG27950.034.2984852
CATGGGT8000.034.0694124
ACATGGG27200.034.0348553
GGTACCT5600.033.5872088
GGGTACC5750.033.5075457
ACGCAGA20550.030.8708487
CGCAGAG21200.029.7215088
TATCAAC22000.029.0497782
GTATAGA1154.126401E-628.6645471
CATGGGG13100.027.2627034
GCAGAGT23350.027.1862169
GGTATCA6450.026.2837721
Produced by FastQC (version 0.11.2)