FastQCFastQC Report
Sun 14 Apr 2019
SRR6576115_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576115_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423750
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16180.1136435469710272No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15630.10978050921861282No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15380.10802458296751538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22950.058.5239451
GGTATCA8400.056.6648251
TCAACGC30600.042.547014
ATCAACG30800.042.272213
CAACGCA31500.041.1821675
AACGCAG32950.039.5125476
TATCAAC38700.034.0073662
ACGCAGA41650.031.1439917
CGCAGAG43850.029.688648
GTACATG56800.029.2885461
TACATGG58950.027.9068092
ACATGGG58900.027.6112923
GCAGAGT49300.026.5019639
AGAGTAC42600.023.1129110-11
CATGGGG35500.023.0373024
ACCTAAG3950.022.668771
CATATAG2458.4910425E-921.1034053
ACATATA2301.00839316E-720.4361042
AGGACCG10900.020.2666535
AGTACAT38350.019.79189312-13
Produced by FastQC (version 0.11.2)