FastQCFastQC Report
Sun 14 Apr 2019
SRR6576116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282726
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15020.11709437557202394No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13550.10563440672442907No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13090.10204829402382114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23100.062.67141
GGTATCA6950.060.1915971
TCAACGC31050.045.5534
ATCAACG31950.044.269823
CAACGCA32800.043.1209035
AACGCAG34000.042.0135966
TATCAAC37350.038.5104332
ACGCAGA41700.034.1430057
CGCAGAG44200.032.211848
GCAGAGT48750.029.5897989
CTTAGGT2800.028.5300833
GTACATG52350.026.4871961
TAGGTAT2900.025.9249045
TACATGG53950.025.3541532
ACATGGG54150.025.0790753
AGAGTAC43150.024.28299710-11
GTCTTAG5600.022.6624261
ATAGGGT2355.2659743E-921.9956573
ACCTAAG3007.2759576E-1221.9349881
GGTATAG3950.021.4126597
Produced by FastQC (version 0.11.2)