FastQCFastQC Report
Sun 14 Apr 2019
SRR6576116_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576116_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282726
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15000.11693845762851926No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14080.10976623222730342No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13940.10867480662277056No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13170.10267196579783991No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13040.10165849916505942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22900.061.8769231
GGTATCA7100.061.6626631
TCAACGC31950.043.6838344
CAACGCA32050.043.5475355
ATCAACG32700.042.9693343
AACGCAG33550.041.7389956
TATCAAC38900.036.966392
ACGCAGA41700.033.4686857
CGCAGAG43950.031.7763278
CTTAGGT2650.030.1465913
GCAGAGT48650.028.8031229
GTACATG53500.026.7496131
TACATGG55300.025.4936072
ACATGGG55150.025.3073183
AGAGTAC42100.024.85158310-11
GTCTTAG4750.024.7767351
CGTATCA1000.001760268123.4966092
TAGGTAT3000.023.4966095
AGGTATA2803.6379788E-1223.4956936
GGTATAG3001.9281288E-1020.3629347
Produced by FastQC (version 0.11.2)