FastQCFastQC Report
Sun 14 Apr 2019
SRR6576117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476966
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT10900.2285278195930108No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9880.20714264748430705No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9640.2021108422822591No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8500.17820976757253137No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG8140.17066205976945947No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA7650.16038879081527824No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA7590.15913083951476625No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC5960.1249564958508573No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT5220.10944176314454279No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT4900.10273268954181221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11150.060.2949681
CAACGCA15100.044.819695
ATCAACG15300.043.6194573
TATCAAC16300.042.1099932
GGTATCA4450.039.0896381
AACGCAG19050.036.5132146
TCAACGC18550.036.4839554
ACGCAGA18750.035.3428157
CGCAGAG19200.034.759258
GCAGAGT19300.034.0921259
GACGTTA1309.167707E-932.5373737
TATTACG753.2733328E-431.3322875
ACGTTAA1351.3242243E-831.3322838
TATAGTG804.7776752E-429.3740165
GTGATCG2300.028.6077388
CTATTGA4200.027.9752569
TACATGG19750.027.8508642
GTATAAG2203.6379788E-1227.7805351
ACATGGG19650.027.7446173
AGGGCTA4300.027.3246675
Produced by FastQC (version 0.11.2)