FastQCFastQC Report
Sun 14 Apr 2019
SRR6576117_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576117_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476966
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9750.20441708633319777No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8980.18827337797662727No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA8810.18470918262517663No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8320.17443591367099542No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7970.16709786441800883No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7540.15808254676433958No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG6330.13271386220401454No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA6290.1318752280036732No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC5700.11950537354863869No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5700.11950537354863869No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC5570.11677981239752938No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC5490.11510254399684675No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA5470.11468322689667607No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5350.11216732429565208No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT5120.10734517764368948No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4970.10420029939240952No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT4910.10294234809189755No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT4890.10252303099172687No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG4840.10147473824130021No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4800.10063610404095889No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC4790.10042644549087357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10150.056.6974951
TAGGGCG250.00523532256.396864
GTACTAG3450.045.1195681
CTAATCC450.001013636741.7754483
GGTATCA4050.040.7645871
CAACGCA14750.038.872415
ATCAACG14850.038.294163
TAGGCAT4500.035.5091325
CGCGCGA352.1854401E-433.56955720-21
TATCAAC17250.033.5111732
AACGCAG18300.032.615676
ACGCAGA17600.032.0436677
CGCAGAG17650.031.9528948
GACGTTA902.5917556E-531.3315877
TTAGGTA600.004159698331.3315854
TATGCTA600.004159698331.3315856
CTAGGCA5450.031.044144
TAGTACT3700.030.4847854
CATCGTC5100.030.4100699
TCAACGC18900.030.3369314
Produced by FastQC (version 0.11.2)