FastQCFastQC Report
Sun 14 Apr 2019
SRR6576118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359271
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8660.2410436689852507No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8090.2251782080936118No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7740.21543625842330721No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7040.195952359082698No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6570.1828703123825747No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC6280.17479841122717948No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA5300.14752095215032662No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC4590.12775871139056588No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT4120.11467666469044259No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT4010.11161490907977543No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA3860.10743978779250205No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3740.10409969076268333No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT3610.10048125231371305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8550.062.666851
CAACGCA12000.046.203945
ATCAACG12150.044.860073
TATCAAC13200.040.9470942
AGGATCG608.8262816E-539.155885
AACGCAG14700.038.9960636
GTACTAG3150.038.7937661
TCAACGC14700.038.3567854
GGTATCA2950.038.23741
GTATTTC757.429182E-637.600111
GTCTAAT500.001700445337.600111
CGCAGAG14900.037.5265778
ACGCAGA15300.036.238387
CTAACGC1302.2919266E-1036.143893
CGTTAAC1204.1891326E-935.2501031
GCAGAGT16150.034.622049
CGCCTAC1658.54925E-1131.3247057
CTATTGA2450.030.6854219
TAATAAT2051.8189894E-1229.796673
TTAACAG1503.6927304E-828.1922343
Produced by FastQC (version 0.11.2)