FastQCFastQC Report
Sun 14 Apr 2019
SRR6576121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643292
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA29730.1809173293608196No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27660.1683206636434669No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23590.1435533064117637No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG21240.1292527438824019No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA20050.12201118243136339No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18600.1131874310834593No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18490.11251804305017002No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18350.11166609464416549No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT18050.10984049091701292No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17920.10904939596858014No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16870.10265978292354615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29700.058.243271
GGTATCA9850.057.266341
ATCAACG37800.045.009593
TCAACGC38200.044.4152534
CAACGCA39600.043.201075
AACGCAG40800.042.0456476
TATCAAC47950.035.977572
ACGCAGA52150.032.804657
CGCAGAG53600.031.9172088
GCAGAGT58450.029.4296349
GTACATG68900.028.0399341
TACATGG69300.027.9459172
CTTAGGT8650.027.7103713
GGTATAG9000.027.677177
ACATGGG70300.027.54423
ACCTAAG11200.026.8606381
AGGTATA9200.026.0537766
TAGGTAT9350.025.63585
GTCTTAG11900.025.2806031
TTAGGTA9750.025.0661164