FastQCFastQC Report
Sun 14 Apr 2019
SRR6576121_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576121_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643292
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30710.18688096820285136No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC27140.16515628384973577No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC23910.14550061705405978No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22230.13527723618200538No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT18880.11489132789546837No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18830.1145870606076096No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18010.1095970770867259No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17860.10868427522314962No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16900.1028423432962614No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16570.10083417919639359No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT16570.10083417919639359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9600.060.8941421
GTATCAA29550.059.0295261
TCAACGC38350.045.465674
ATCAACG38900.044.9450263
CAACGCA39900.043.817255
AACGCAG41300.041.8767436
TATCAAC49300.035.654392
ACGCAGA52600.032.8804057
CGCAGAG54800.031.3040568
GTCTTAG12000.031.036371
TAGGTAT8950.030.4565035
CTTAGGT8450.030.0348423
GCAGAGT58250.029.530689
TTAGGTA9150.028.2498844
GTACATG68750.027.4977231
TACATGG69150.027.0507412
ACATGGG69200.026.8274443
GGTATAG9550.026.5745227
AGGTATA9400.026.498616
GGTAATC3350.025.2531788