FastQCFastQC Report
Sun 14 Apr 2019
SRR6576122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464823
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28070.19162724779717413No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC26420.18036308823659924No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22950.15667421934254172No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20550.14028998725443279No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18020.12301827592821794No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17810.12158465562050842No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT16780.11455308934936166No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16680.11387041301235712No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15700.10718018490971264No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15650.10683884674121039No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT15160.10349373268988814No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15150.10342546505618767No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14730.1005582244407686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.058.1238981
GTATCAA27000.056.7536351
TCAACGC35300.042.465594
ATCAACG36100.041.3943563
CAACGCA36900.040.621495
AACGCAG38050.039.3937726
TATCAAC45950.033.3481642
TAGGTAT7950.031.9167145
ACGCAGA47100.031.8233957
ACCTAAG9750.031.8184591
GTATACG753.2741213E-431.3363591
CTTAGGT8200.030.9457573
CGCAGAG49100.030.622828
TTAGGTA8300.030.006754
AGGTATA8100.029.585356
GTACATG59300.029.0905021
GGTATAG8350.028.698587
GTCTTAG10200.028.5713861
CTAAGAC12600.028.34423
GCAGAGT53300.028.2097669
Produced by FastQC (version 0.11.2)