FastQCFastQC Report
Sun 14 Apr 2019
SRR6576122_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576122_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464823
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30140.20575864797316806No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC25310.17278538089584883No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC22290.15216855551831177No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20500.13994864908593052No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT17660.1205606411150016No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16930.11557710385486847No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16790.11462135698306211No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16780.11455308934936166No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15560.10622443803790628No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14680.10021688627226634No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT14660.10008035100486544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25900.061.6186031
GGTATCA8550.051.7575381
ATCAACG34950.044.237343
TCAACGC34900.044.1660654
CAACGCA36100.042.5677645
AACGCAG37350.041.018726
TATCAAC43350.036.208662
ACGCAGA48150.031.7195747
CGCAGAG50050.030.630248
CTTAGGT6400.030.1053833
AGGTATA6250.030.0770426
GTCTTAG8100.029.6412871
GTACATG61450.028.8822731
TTAGGTA6450.028.4148374
GCAGAGT54700.028.1123629
TACATGG62450.027.8435362
GGTATAG6850.027.4416167
TAGGTAT6900.027.2427655
ACATGGG63050.027.055893
ACCTAAG9650.026.8316441