FastQCFastQC Report
Sun 14 Apr 2019
SRR6576123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458206
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA72970.5004094071756665No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC69640.47757312752793496No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC64110.4396498162811016No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT33210.2277455997300793No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG33000.22630547398652867No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG28720.1969543397846395No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC24800.17007199257169425No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG23190.15903102853780604No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC21900.15018454182742358No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT21880.15004738699470446No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA20070.13763487463362517No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT19840.1360575940573554No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT19730.13530324247740033No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA19010.13036566849951242No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT18960.13002278141771464No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA18220.12494805260710763No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA17180.11781600130571401No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC16650.11418139823865764No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT15310.10499202444647739No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA15190.10416909545016273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30200.057.4320761
GGTATCA9650.054.5538981
TCAACGC39350.043.475754
CAACGCA39750.042.9200175
ATCAACG39950.042.5875053
AACGCAG42150.040.8106886
TATCAAC44000.039.0988042
CTTAGGT17950.036.394923
TTAGGTA18250.035.7966464
TAGGTAT18000.035.5105065
AGGTATA18000.035.2493976
GTCTTAG20750.034.205341
ACGCAGA51000.033.3602147
GGTATAG18950.033.234267
CGCAGAG51600.033.063398
TCTTAGG21150.031.7805022
ACCAGAT25200.030.58677794
GTATAGT21050.029.6954528
GCAGAGT58300.029.5861159
GTACATG68200.029.4292071
Produced by FastQC (version 0.11.2)