FastQCFastQC Report
Sun 14 Apr 2019
SRR6576123_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576123_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458206
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA78560.5387441829206573No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC68290.4683151763193952No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC64290.4408842097755735No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG31910.21883053560333726No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT30670.21032693597475252No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG23740.16280278643758153No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG22280.15279048364908662No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC21510.14751002258940094No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT21460.1471671355076032No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC21330.14627562909492897No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT20960.14373826468962547No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT19550.13406884898292834No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA19000.13029709108315285No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT18760.12865123309052356No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA17600.12069625279281528No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA16460.1128784273278261No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA15620.10711792435362356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9150.060.76151
GTATCAA29000.058.6511881
ATCAACG38000.043.6631363
TCAACGC38050.043.6057624
CAACGCA39600.041.6601565
TATCAAC40900.040.9119832
AACGCAG41300.040.0591356
GGTAATC4850.036.8231548
TTAGGTA19850.036.4656564
AGGTATA20150.036.154776
TAGGTAT20150.036.154775
GTCTTAG21450.035.80371
CTTAGGT20000.035.7221373
ACGCAGA48250.033.9943927
GGTATAG21600.033.5078167
GTACTAG2250.033.504651
CGCAGAG49750.032.9694378
TCTTAGG22100.032.965772
ACCAGAT24850.032.3428894
GTATAGT23200.031.8046658