FastQCFastQC Report
Sun 14 Apr 2019
SRR6576124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319979
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA71780.5437965300963121No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC63230.4790227723319841No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC63200.4787954959889513No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG31530.23886743652739928No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT31420.23803408993627928No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG27500.20833664778000258No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC23730.1797755873388895No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC21570.16341169064053293No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG21050.1594722340279656No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT19420.14712355272318728No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT19330.14644172369408906No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA19250.1458356534460018No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA18930.14341137245365268No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT18330.13886584559299808No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT17940.13591125313357258No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA17630.13356273092223436No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA16760.1269717169742852No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC15980.12106253205543421No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA14430.10931992099874316No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT14120.10697139878740494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28950.054.715651
GGTATCA10350.053.13441
ATCAACG37450.040.524383
TCAACGC38250.039.6768154
CAACGCA38850.039.1849825
AACGCAG40400.038.0305066
TATCAAC41400.037.6936342
TAGGTAT18050.035.9225855
AGGTATA17700.035.836556
CTTAGGT18050.035.6622773
TTAGGTA18500.034.540844
GTCTTAG20650.033.6877441
GGTATAG19150.033.1230777
TCTTAGG20900.032.610092
ACGCAGA47450.032.1819887
GTATAGT20050.032.1049428
ACCTAAG23150.031.064951
GGTAATC5150.031.0196868
CGCAGAG49350.030.9429638
ACCAGAT22850.030.63837694
Produced by FastQC (version 0.11.2)