FastQCFastQC Report
Sun 14 Apr 2019
SRR6576124_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576124_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319979
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA75420.5713727263842834No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC66050.5003867485770608No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC61040.4624315992905948No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT30650.2322006637984392No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG30160.22848848352890463No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG22830.17295729704790758No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG22340.16924511677837298No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC20570.15583581253944193No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC19840.15030542152564547No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT19760.1496993512775582No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT19480.1475781054092527No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA18730.14189619683343446No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT18190.13780522265884534No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA17540.13288090189313617No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT16500.12500198866800155No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA16410.12432015963890335No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA15000.11363817151636504No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC13890.10522894682415403No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT13270.10053190240147761No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT13220.10015310849642305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26150.060.1562581
GGTATCA7650.056.64121
TCAACGC34950.044.7745634
ATCAACG35450.043.8779223
CAACGCA35900.043.589725
TATCAAC37700.041.510092
AACGCAG38650.040.8514636
ACGCAGA45650.034.5872767
TAGGTAT14850.034.493275
CGCAGAG46650.033.7681478
CTTAGGT15650.032.7300383
GTCTTAG17800.032.280921
TTAGGTA15700.032.027164
AGGTATA16000.031.4254636
TCTAACG902.5961355E-531.3299392
TCTTAGG17600.030.9739152
GTACATG61200.030.6293451
GCAGAGT51800.030.2293329
GTACTAG1252.3207213E-730.1429711
ACCAGAT25300.030.11213194
Produced by FastQC (version 0.11.2)