FastQCFastQC Report
Sun 14 Apr 2019
SRR6576127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852154
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12100.054.75281
GGTATCA4650.048.502141
TCAACGC16300.039.7799384
ATCAACG16200.039.735453
CAACGCA17050.038.0300875
AACGCAG17450.037.1583376
TATCAAC19900.032.819682
ACCTAAG2850.028.0270021
ACGCAGA23150.027.6032717
GTACATG32300.026.7662721
CGCAGAG24300.026.2969448
GTATAGG2008.1490725E-1025.8425451
TACATGG33200.025.4745792
ACATGGG33700.024.678343
GCAGAGT27400.023.836199
GCTTAGG1000.001761261323.4932231
TGTACCG1602.0428906E-623.4932235
CTAAGAC5600.023.4932213
TAATACC1852.8208342E-722.8582734
TAAGACA6200.022.7353764
Produced by FastQC (version 0.11.2)