FastQCFastQC Report
Sun 14 Apr 2019
SRR6576127_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576127_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852154
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9740.11429858922213591No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9340.1096046019850872No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9130.10714025868563665No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8610.10103807527747331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14250.063.3913421
GGTATCA4100.055.080891
ATCAACG19250.045.1621483
TCAACGC19500.044.824144
CAACGCA19850.044.0337875
AACGCAG20800.042.2485546
TATCAAC22000.039.7304882
ACGCAGA26450.033.046157
GTACCGT600.00416339631.3287016
TTACTCG600.00416339631.3287014
CGCAGAG28000.031.2388068
GCAGAGT29550.029.7593639
TATACGA650.006157736328.9187972
CTTAGGT2450.028.7712543
GTATACG856.773865E-427.6754471
GTACATG33750.026.9046341
CGACGTT350.00884357726.85316734-35
TACATGG34200.026.2446562
ACATGGG34100.025.7703823
GTGTAAT1859.920768E-925.4314921
Produced by FastQC (version 0.11.2)