FastQCFastQC Report
Sun 14 Apr 2019
SRR6576128_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576128_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175344
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15450.1314508773601601No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13550.11528539729645107No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13390.11392409371213874No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG12620.10737282021263561No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12440.10584135368028424No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG12320.10482037599204999No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11900.1012469540832301No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11870.10099170966117153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22400.060.387631
GGTATCA7400.056.0486371
ATCAACG30950.043.1271363
TCAACGC30950.043.1271364
CAACGCA31850.042.054255
AACGCAG32250.041.241196
TATCAAC37950.034.9245072
ACGCAGA40450.032.648437
CGCAGAG43350.030.5727488
GCAGAGT45050.029.6277059
GTACATG53300.028.1199191
TACATGG54700.027.3232752
ACATGGG55250.026.6259423
AGAGTAC39700.025.74798210-11
ACCTAAG3950.025.0574171
GTCTTAG5200.023.5659071
TAAGGGT2202.4519977E-923.4997334
CTAAGAC7150.023.0067313
CATGGGG30650.022.0813164
ATGGGAG13400.021.0436425
Produced by FastQC (version 0.11.2)